Atto488-LPETGG

£125.00 0.1mg

Sortase reagent.

Atto488-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label Atto488. Atto488-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. Atto488-LPETGG contains the Atto488 fluorescent label which is efficiently excited in the range 480 – 515 nm, with strong absorption, high fluorescence quantum yield and high photo-stability.

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SKU: FP-010 Categories: ,

Additional information

Three Letter Sequence

Atto488-Leu-Pro-Glu-Thr-Gly-Gly-OH

Molecular Weight

1144.6

Molecular Formula

C49H62N9O19 S2

Sequence

Atto488-LPETGG

Solubility

Soluble in water

Appearance

Freeze dried solid

Storage

Storage desiccated, frozen and in the dark

Purity

>95% by HPLC

Modifications

Atto488 label at N terminal

Searchable Words

Atto488-LPETGG, sortase, LPETGG, fluorescent label, Atto488, transpeptidation, FP-010, FP010