Asymmetrical Dimethylarginine (ADMA) BioAssay ELISA Kit

£1,111.00 96 Tests

Asymmetrical Dimethylarginine (ADMA) BioAssay ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ADMA in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US023601 Categories: ,

Additional information

Detection Range

12.35-1000ng/ml

Sensitivity

4.42ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023601A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*023601B: Standard, 2×1 vial
023601C: Standard Diluent, 1x20ml
*023601D: Detection Reagent A (green), 1x1vial
*023601E: Detection Reagent B (red), 1x120ul
023601F: Assay Diluent A, 1x12ml
023601G: Assay Diluent B, 1x12ml
023601H: TMB Substrate, 1x9ml
023601K: Stop Solution, 1x6ml
023601L: Wash Buffer, 30X, 1x20ml
023601M: Reagent Diluent. for 023601D, 1x300ul

Precaution

The Stop Solution (023601K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023601A, *023601B, *023601D and *023601E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months