Apoptosis Signal Regulating Kinase 1 (ASK1) BioAssay ELISA Kit (Human)

£990.00 96 Tests

The Human Apoptosis Signal Regulating Kinase (ASK1) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ASK1 in human tissue homogenates and other biological fluids.

SKU: US023545 Categories: ,

Additional information

Detection Range

0.312-20ng/ml

Sensitivity

< 0.115ng/ml

Precision

Intra-Assay: CV<10%
Inter-Assay: CV<12%

Test Principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ASK1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ASK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ASK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of ASK1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Kit Components

*023545A: Microtiter Plate, 1×96 wells, Pre-coated; ready to use
*023545B: Standard, 2x1vial
023545C: Standard Diluent, 1x20ml
*023545D: Detection Reagent A (green), 1x120ul
*023545E: Detection Reagent B (red), 1x120ul
023545F: Assay Diluent A, 1x12ml
023545G: Assay Diluent B, 1x12ml
023545H: TMB Substrate, 1x9ml
023545K: Stop Solution, 1x6ml
023545L: Wash Buffer, 30X, 1x20ml

Storage and Stability

Store *023545A, *023545B, *023545D and *023545E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Shelf Life

6 Months