Apolipoprotein E (APOE) BioAssay ELISA Kit (Mouse)

£976.00 96 Tests

Apolipoprotein E (APOE) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of APOE in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US023530 Categories: , ,

Additional information

Detection Range

15.6-1000ng/ml

Sensitivity

6.9ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023530A: Microtiter Plate, 1×48 wells or 1×96 wells
*023530B: Standard, 2x1vial
*023530C: Detection Reagent A (green), 1x60ul or 1x120ul
*023530D: Detection Reagent B (red), 1x60ul or 1x120ul
023530E: TMB Substrate, 1×4.5ml or 1x9ml
023530F: Wash Buffer (30X), 1x10ml or 1x20ml
023530G: Standard Diluent, 1x10ml or 1x20ml
023530H: Assay Diluent A, 1x6ml or 1x12ml
023530J: Assay Diluent B , 1x6ml or 1x12ml
023530K: Stop Solution, 1x3ml or 1x6ml
*023530L: Positive Control, 134.7ng/ml, 1x1vial

Precaution

The Stop Solution (023530K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023530A, *023530B, *023530C, *023530D and *023530L at -20?C. After reconstitution of *023530L, store at 4?C and use within 5 days Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 023530L

Positive Control

Intended Use

The control vials are from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vials contain mouse APOE in mouse serum (lyophilized powder). Note: The mouse based serum used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).

Expected Value

134.7ng/ml

Usage

Dissolve with 150ul standard diluent and let stand for 10min. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Source Antigen

Mouse

Shelf Life

6 Months