Apolipoprotein A4 (APOA4) BioAssay ELISA Kit (Human)

£990.00 96 Tests

Apolipoprotein A4 (APOA4) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of APOA4 in human serum, plasma and other biological fluids.

SKU: US023500 Categories: , ,

Additional information

Detection Range

78-5000pg/ml

Sensitivity

34pg/ml

Precision

Intra-Assay:
CV <10%

Inter-Assay:
CV <12%

Kit Components

*023500A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*023500B: Standard, 2×1 vial
023500C: Standard Diluent, 1x20ml
*023500D: Detection Reagent A (green), 1x120ul
*023500E: Detection Reagent B (red), 1x120ul
023500F: Assay Diluent A, 1x12ml
023500G: Assay Diluent B, 1x12ml
023500H: TMB Substrate, 1x9ml
023500K: Stop Solution, 1x6ml
023500L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (023500K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023500A, *023500B, *023500D and *023500E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Shelf Life

6 Months