Angiopoietin Like Protein 4 (ANGPTL4) BioAssay ELISA Kit (Human)

£990.00 96 Tests

Angiopoietin Like Protein 4 (ANGPTL4) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANGPTL4 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US023384 Categories: , ,

Additional information

Detection Range

0.78-50ng/ml

Sensitivity

0.31ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023384A: Microtiter Plate, 1×48 wells or 1×96 wells, Pre-coated, ready to use
*023384B: Standard, 2×1 vial?
023384C: Standard Diluent, 1x10ml or 1x20ml
*023384D: Detection Reagent A (green), 1x60ul or 1x120ul
*023384E: Detection Reagent B (red), 1x60ul or 1x120ul
023384F: Assay Diluent A, 1x6ml or 1x12ml
023384G: Assay Diluent B, 1x6ml or 1x12ml
023384H: TMB Substrate, 1×4.5ml or 1x9ml
*023346J: Positive Control, 11.68ng/ml, 1x1vial
023384K: Stop Solution, 1x3ml or 1x6ml
023384L: Wash Buffer, 30X, 1x10ml or 1x20ml

Precaution

The Stop Solution (023384K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023384A, *023384B, *023384D, *023384E and *023384J at -20?C. After reconstitution of *023384J, store at 4?C and use within 5 days Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 023384L

Positive Control

Intended Use

The control vial is from a human serum sample known to contain ANGPTL4. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains human ANGPTL4 in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values

11.68ng/ml

Usage

Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.
Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.?

Shelf Life

6 Months