Amyloid Precursor Protein, Human (APP) BioAssay ELISA Kit

£1,291.00 96 Tests

SKU: USA2275-69L Categories: ,

Additional information

Sensitivity

<0.4ng/ml The analytical sensitivity of this assay is <0.4ng/ml of Human APP. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 30 times.

Specificity

The Human APP BioAssay? ELISA kit is specific for measurement of total APP protein. The following proteins were tested at 100ng/ml and found to have no crossreactivity: Tau, a-synuclein, b-synuclein, Ab1-40 and Ab1-42.

Range

0.78-50ng/ml

Incubation Time

4 hours

Sample Size

10ul (diluted to a total volume of 100ul)

Sample Type

Cell lysates and cerebral spinal fluids

Specifications

Simple: Pre-coated strip-well plates.
Liquid stable chromogen and stop reagents.
Economical: One 96-well plate, plus reagents for 96 determinations.
Reagents are stable for multiple runs.

Precision

Intra-assay: <8%
Inter-assay: <9%

Kit Components

*A2275-69L1: Standard, 2×1 vial
Supplied as a lyophilized powder. Reconstitute with 1ml of A2275-69L2: Standard Diluent Buffer.
A2275-69L2: Standard Diluent Buffer,1x25ml
Supplied as a liquid containing 8mM sodium azide.
A2275-69L3: Microtiter Plate, 1×96 wells
A2275-69L4: anti-APP (Biotin), 1x11ml
A2275-69L5: Streptavidin (HRP) (100X), 1x125ul
Supplied as a liquid containing 1.7mM thymol.
A2275-69L6: Streptavidin (HRP) Diluent, 1x25ml
Supplied as a liquid containing 1.7mM thymol and 0.05% Proclin 300.
A2275-69L7: Wash Buffer (25X), 1x100ml
A2275-69L8: TMB, 1x25ml
A2275-69L9: Stop Solution, 1x25ml

Storage and Stability

Store *A2275-69L1: Standard powder at 4°C, liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Temperature

On Dry Ice

Source Antigen

Human

Shelf Life

6 Months