Albumin (ALB) BioAssay ELISA Kit (Rat)

£770.00 96 Tests

Albumin (ALB) BioAssay ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ALB in rat serum, plasma and other biological fluids.

SKU: US023210 Categories: , ,

Additional information

Detection Range

2.47-200ug/ml

Sensitivity

0.94ug/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023210A: Microtiter Strips, 1×48 wells or 1×96 wells
*023210B: Standard, 2×1 vial
023210C: Standard Diluent, 1x10ml or 1x20ml
*023210D: Detection Reagent A, 1x60ul or 1x120ul
*023210E: Assay Diluent A, 1x6ml or 1x12ml
023210F: TMB Substrate, 1×4.5ml or 1x9ml
023210G: Stop Solution, 1x3ml or 1x6ml
023210H: Wash Buffer, 30X, 1x10ml or 1x20ml
*023210J: Positive Control, 104.1ug/ml, 1×1 vial

Precaution

The Stop Solution (023210G) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023210A, *023210B, *023210D, and *023210J at -20?C. After reconstitution of *023210J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 023210J

Positive Control

Intended Use

The control vials are from rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vials contain rat ALB in rat serum (lyophilized powder). Note: The rat based plasma used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).

Expected Values

104.1ug/ml

Usage

Dissolve with 150ul standard diluent and let stand for 10min. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
5. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months