Ac-DEVD-AMC
£49.00 1mg
Fluorogenic substrate for caspase-3.
Ac-DEVD-AMC is a fluorogenic substrate for caspase-3 with a Km value of 9.7 1.0 μM. The sequence of Ac-DEVD-AMC is based on the PARP cleavage site Asp216 for caspase-3 and it is also a substrate for caspases -1, -4, -7 and -8. Ac-DEVD-AMC can be used to monitor intracellular caspase activity with cleaved AMC moieties, reflecting caspase activity, being detected by fluorescence.
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Additional information
| Three Letter Sequence | Ac-Asp-Glu-Val-Asp-AMC |
|---|---|
| Molecular Weight | 675.7 |
| Molecular Formula | C30H37N5O13 |
| Sequence | DEVD |
| Solubility | Soluble in DMSO; dilute with buffer for use |
| Appearance | Lyophilised solid |
| Storage | We recommend storage desiccated, frozen and in the dark |
| Purity | ‰¥97% (HPLC) |
| Modifications | Acetyl at N terminal, 7-amino-4-methylcoumarin (AMC) at C terminal |
| Searchable Words | DEVD, substrate, caspase-3 , 169332-61-0, Ac-DEVD-AMC, PI-260, PI260 |