5-FAM-LPETGG

£125.00 0.1mg

Sortase reagent.

5-FAM-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label 5-FAM. 5-FAM-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. 5-FAM-LPETGG contains the a 5- carboxyfluorescein label, which is one of the most widely used green fluorescent reagents for in situ labeling of peptides, proteins and nucleotides.

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SKU: FP-020 Categories: ,

Additional information

Three Letter Sequence

5-FAM-Leu-Pro-Glu-Thr-Gly-Gly-OH

Molecular Weight

930.83

Molecular Formula

C45H50N6O16

Sequence

5-FAM-LPETGG

Solubility

Soluble in water

Appearance

Freeze dried solid

Storage

Store desiccated, frozen and in the dark

Purity

>95% by HPLC

Modifications

5-FAM at N terminal

Searchable Words

5-FAM-LPETGG-OH, 5-FAM-LPETGG, 5-FAM LPETGG, sortase, fluorescent label, FP-020, FP020